Evaluation of free radical scavenging activity of various leaf extracts from. Activity was calibrated using a standard material, lascorbic acid 23,4dihydro2,5,7,8. Superoxide radical scavenging agents in treatment of. Antioxidant, free radical scavenging and antibacterial. Preparation and evaluation of antioxidant activities of. A comparative study on the antioxidant activity of. The results suggested that dwgps produced by fermentation could be used as a promising antioxidant ingredient. To test the hypothesis that superoxide radicals are involved in tissue destruction after alkali burns, superoxide dismutase, ascorbic acid, and glutathione were used as superoxide radical scavenging agents. However, there has been no report on the antioxidant properties of this species. Dec 09, 2008 the objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s.
Pdf hydrogen peroxide radical scavenging and total. Takeki hamasaki, taichi kashiwagi, toshifumi imada, noboru nakamichi, shinsuke aramaki, kazuko toh shinkatsu morisawa, hisashi shimak oshi, yoshio hisaeda, and sanetaka shirahata. Assay of nitric oxide scavenging activity nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern. The plant extracts exhibited strong antioxidant and radical scavenging activity on abts radical cation, dpph free radical, hydrogen peroxide. Antioxidant methods used were hydrogen peroxide scavenging h 2 o 2 assay and superoxide radical scavenging activity sod.
For determination of radical scavenging activity of different foods, beverages and substrates were elaborated a. Free radical scavenging activity of phellinus merrillii 409 abts free radical scavenging assay total antioxidant status of the pm was measured using 2, 2. The present study evaluated the in vitro antioxidant and antibacterial activities of various extracts of aerial parts of periploca aphylla and ricinus communis. Therefore, in presence of superoxide desmutase that uses superoxide anion radicals as substrate, one would expect decrease in the formation of nitrite, formazan or reduction of cytochrome c. Assay for superoxide radical scavenging activity was based on the capacity of the sample to inhibit blue formazan formation by scavenging the superoxide radicals generated in riboflavinlightnbt system 5,10,12. Herbs and pastures, superoxide anion radical, scavenging activity, esr, hplc, altitudinal locations. Total antioxidant activity, metal chelation, radical scavenging dpph effects and reducing power as well as activities destructive to active oxygen species such as the superoxide anion radical, hydroxyl radical, and hydrogen peroxide are widely used for this purpose shimada et al. Dpph radical scavenging assay dpph radical scavenging activity was measured as per standard method using stable dpph radical7. The ic50 value table 1 of the test sample was centage inhibition of deoxyribose degradation. The antioxidant activity of the aerial part extract of m. Superoxide scavenging activity of the plant extract was determined by mccord and fridovich method, 1969 6 which depends on light induced superoxide generation by riboflavin and the corresponding reduction of ni troblue tetrazolium was measured spectrophotometrically at 560 nm. In vitro free radical scavenging and antidiabetic activity of. Embelin as a scavenger of superoxide is stronger than the common food additive antioxidant 2,6bis1,1dimethylethyl420 methylphenol, butylated hydroxytoluene, bht. Superoxide anion radical scavenging activity of cassia.
The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11. Dpph free radical scavenging activity of the extracts of the. What is the mechanism of superoxide anion free radical. Beyza ozturk sarikaya and ilhami gulcin affiliation. The raw african yam bean seed was dry heated in air oven at 100. The ec 50 values for the scavenging rates of dpph, hydroxyl and superoxide anion radicals were 3. Eggplant epidermis extract exhibited the most potent hydroxyl radical scavenging and sodlike activities. An attempt was made to investigate the antioxidant activity of the methanolic extract of diplazium esculentum.
Abstractthe present study was designed to explore the flavonoid contents and. The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxidescavenging of other antioxidants. Heat treatments application affected the phenolic contents of the seeds significantly p radical scavenging activity of the phenolics were done using 2,2diphenyl 1picrylhydrazyl dpph. Scavenging of hydroxyl radical was determined using dmpo. Free radical scavenging activities of watersoluble extracts from some natural sources. They showed a varying degree of dpph radical scavenging activity with ic50 values between 31. In vitro antioxidant activities of the plant extract were determined by dpph and no scavenging method. In vitro antioxidant and free radical scavenging activity.
The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxide scavenging of other antioxidants. Cucurbitaceae is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Invitro antioxidant and free radical scavenging activity. Data obtained showed that the extract scavenged superoxide anion radicals ic 501602 gml in a dose dependant manner using. Reactive oxygen species ros, including free radicals such as superoxide anion radicalso. Free radical scavenging activity of seed of holarrhena. A part of the oxygen taken into living cells is changed to several harmful reactive oxygen species and free radicals.
Scavenging activities of these plants are comparable with standards. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents. Reactive oxygen species ros, including the free radicals superoxide anion o 2. Superoxide radical scavenging properties of extracts and. A superoxide is a compound that contains the superoxide ion, which has the chemical formula o. The superoxide anion scavenging activity or sod like activity of mum212 strain was examined using an indirect colorimetric method 19160 sod assay kitwst, sigma aldrich that measures the formation of water soluble formazan dye upon the reduction of 24iodophenyl34nitrophenyl52,4disulfophenyl2htetrazolium, monosodium salt wst1 by. Invitro antioxidant and free radical scavenging activity of. Free radical scavengers definition of free radical. A blank solution containing phosphate buffer, without h2o2 was prepared.
Serotonin, antioxidant activity, metal chelating, reducing power, radical scavenging. A comparative study on the antioxidant activity of methanolic. The method dpph is widely used for measurement of free radical scavenging ability of antioxidants perezjimenez and sauracalixto, 2008. Scavenging activity on dpph radical quantitative fig. Evaluation of abelmoschus moschatus extracts for antioxidant. Radical scavenging and antioxidant capacity of serotonin volume. Antioxidant and free radical scavenging activities of. Antioxidant and free radical scavenging potential of citrullus colocynthis l. Superoxide anion radical scavenging activity biology essay. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500.
Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was. The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7. Superoxide definition of superoxide by medical dictionary. Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability. In vitro evaluation of free radical scavenging activity of.
Ataturk university, faculty of sciences, department of chemistry, 25240erzurum, turkey. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity. Aug 17, 2011 superoxide radical scavenging activity. Hydrogen peroxide is also damaging and is degraded by other. Superoxide anion radical scavenging activity superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Relationships between free radical scavenging and antioxidant. Superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Frsa free radical scavenging activity acronymfinder. This article is from iranian journal of pharmaceutical research. Superoxide anion radical scavenging activities of herbs and. Antioxidant and free radical scavenging potential of. Preliminary phytochemical screening of the plant showed the presence of large amounts of. Chrysin, rutin and quercetin were run to explore the effect of.
In this study we determined the free radical scavenging activity, total phenolic content, total oxidant level, and total antioxidant level in methanol and acetone extracts of t. Antioxidants free fulltext antioxidant properties of. Evaluation of free radical scavenging activity of an. Antioxidant, antimicrobial, and free radical scavenging. In vitro antioxidant activities, free radical scavenging. Chemical composition and radical scavenging activity of. It is evident from the data that periploca aphylla and ricinus communis possess hydrogen donating capabilities and. Antioxidant and free radical scavenging activity of. Free radical scavenging activity, total phenolic content.
In vitro nitric oxide scavenging activity of methanol. In vitro free radical scavenging and antidiabetic activity. Table 1 shows the percentage of dpph radical scavenged by standards, that is, npropyl gallate and 3tbutyl4hydroxyanisole and different extracts of 2 plants at various concentrations. This study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of cassia siamea and cassia javanica by superoxide anion radical scavenging assay. Free radical scavenging activity of crude extracts and 4. In addition, aafb melanin exhibited stronger scavenging activities on 2,2diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. Free radical scavenging activity of methanolic extract of. Acylhydrazide schiff bases 127 were evaluated for their in vitro dpph radical and superoxide anion scavenging activity. The radical scavenging activity, antioxidant activity by carotene bleaching assay and, superoxide dismutase sod like ability decreased in the order of 70% methanol, 70% ethanol, cm, ea and, n. Radical scavenging and antioxidant capacity of serotonin. Radical scavenging and antioxidant activity of tannic acid. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. An aqueous extract of this plant inhibits the citrus canker of lime 8.
In vitro antioxidant and free radical scavenging activity of. Comparative study of abts radical scavenging activity and. Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186. Superoxide anion radical scavenging assay the method of ganga et al. The reactive oxygen ion superoxide is particularly important as the product of the oneelectron reduction of dioxygen o 2, which occurs widely in nature. The antioxidant activity of the memq was evaluated by the. By using hydrodynamic voltammetry, which generates the superoxide radical in situ, we show an unusual scavenging capability by embelin.
Hydroxyl and superoxide anion radical scavenging activities of. Antioxidant and free radical scavenging activity of spondias. Blumea balsamifera, flavonoids, leaf extracts, superoxide radical. The different extracts showed significant inhibition of superoxide radicals in a dosedependant manner. To compare the antioxdant activity we used abts 2,2azinobis 3ethylbenzothiazoline6sulfonic acid diammonium radical scavenging assay which is applicable for both lipophilic and hydrophilic antioxidants. Free radical scavenging activity of the methanol extract was tested in three in vitro models, viz. The objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. There are numerous antioxidant methods for evaluation of antioxidant activity. The reaction mixture contained 50 mm phosphate buffer ph 7.
Dpph free radical scavenging activity of the extracts of. The total phenolic content of the extracts was found to be higher in a. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Superoxide anion radical scavenging activities of herbs. These results suggest that tpl had the highest radical. The antioxidant activity of the aqueous ethanolic extract of doum leaves, hyphaene thebaica l. Superoxide scavenging activity 10 superoxide anions were generated in samples that contained in 3. Radical scavenging effects of different veronica species. Sodlike supreme was found to possess potent free radical scavenging activity in vitro as assessed by an oxygen radical absorbance capacity assay. Oxygen radical scavenging activity of flavonoidschemistry, metabolism, cardioprotective effects, and dietary sources.
The superoxide anion scavenging activity or sod like activity of mum212 strain was examined using an indirect colorimetric method 19160 sod assay kitwst, sigma aldrich that measures the formation of water soluble formazan dye upon the reduction of 24iodophenyl34nitrophenyl52,4disulfophenyl2htetrazolium, monosodium salt wst1. Similarly, the superoxide free radical assay revealed the scavenging activity of eeap, eemd, and aemd which is comparable with standard ascorbic acid whereas aeap displayed significantly less scavenging activity over other tested extracts and standard fig. Several methods have been developed to assess the radical scavenging activity. Free radical scavenging activity of crude extracts and 4 bioline. Molecular oxygen dioxygen is a diradical containing two unpaired electrons, and. Determination of free radical scavenging activity of plant. Hydroxyl radical scavenging activity hrsa of the extractives was determined by the method of klein et al. The percentages of radical scavenging property of the ethanolic, aqueous extract and parthenolide in this assay were 63. Moreover, it had scavenging activity on hydroxyl radical compared with ascorbic acid fig. In the present study, methanolic fruit extract of c. The antioxidant activity of the tannic acid, bha, bht.
In this study, the influencing factors have been systematically investigated for the first time, and a number of experiments have proved that the ph is. In addition, aafb melanin exhibited stronger scavenging activities on 2,2 diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. Superoxide anion and hydroxyl radical scavenging activities of. It was suggested that their higher activity on superoxide anion and dpph free radical scavenging activity, reducing capacity and inhibition of lipid peroxidation seem to be related with the release of specific antioxidants such as low molecular weight protein fragment, peptide and free amino acid derived form chicken leg bone protein during heating treatment rather than the increase of peptide. Flavonoid contents and free radical scavenging activity of. The superoxide radical scavenging activity was measured by the method of nishikimi et al.
Daily subconjunctival injections were given in rabbit eyes after alkali burns. Dpph radical scavenging capacity of phenolic extracts from. Once formed, free radicals can start a chain reaction leading to formation of more free radicals. Superoxide anion radical activity was measured by the. Three extracts of seed amsi, amsii and amsiii and one leaf extract amli were found to be an efficient scavenger of superoxide radical generation. Superoxide is produced as a byproduct of oxygen metabolism and, if not regulated, causes many types of cell damage. Nov, 2009 this study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of cassia siamea and cassia javanica by superoxide anion radical scavenging assay. Superoxide radical scavenging activity by alkaline dmso method superoxide radical scavenging activity of the extracts were determined by alkaline dmso method according to the method of elizabeth and rao 1990 with slight modification in this method. Freeradical scavenging properties of low molecular weight. Abts was dissolved in deionized water to 7 mm concentration, and potassium.
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